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【文献标题】Effects of group size on the behaviour, heart rate, immunity, ａnd growth of Holstein dairy calves
【作者】Jing Lv , Xun-Wu Zhao et.al
【作者单位】东北农业大学（Northeast Agricultural University）
【文献中引用产品】
牛白细胞介素2（IL-2）ELISA试剂盒
牛白细胞介素6(IL-6)ELISA试剂盒
牛分泌型免疫球蛋白A(sIgA)ELISA试剂盒
牛肿瘤坏死因子α（TNF-α）ELISA试剂盒
【关键词】Calf,Group size,Behaviour,Immunity,Growth,Animal welfare
【DOI】https://doi.org/10.1016/j.applanim.2021.105378
【出版期刊】《Applied Animal Behaviour Science》
【产品原文引用】
The contents of SIgA (E-78235) in saliva ａnd serum IL-2 (E-80069), IL-6 (E-80071), ａnd TNF-α (E-80095) were determined using commercial ELISA test kits (Shanghai Jinma Laboratory Equipment Corporation., Ltd., Shanghai, China, LOT201410) following the manufacturer’s instructions. Briefly, captured antibodies against bovine SIgA or bovine IL-2, IL-6, ａnd TNF-α were coated on plate wells. The serum ａnd saliva samples were incubated with the solution ａnd then washed to remove nonspecific binding. Horseradish peroxidase bovine anti-bovine SIgA or IL-2, IL-6, ａnd TNF-α were used as detection antibodies. Optical density was recorded at 450 nm with a microplate reader (Bio-tek Instrument Inc., USA). Standard curves drawn from the concentration ａnd standard absorbance were used to quantify the saliva SIgA ａnd serum IL-2, IL-6, ａnd TNF-α levels.
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