【文献标题】Lentivirus expressing soluble ST2 alleviates bleomycin-induced pulmonary fibrosis in mice 【作者】Qiaoyan Gao, Yan Li,Xiuhe Pan,et.al 【作者单位】宁波大学(Ningbo University) 【文献中引用产品】 小鼠羟脯氨酸(Hyp)elisa试剂盒 【关键词】Interleukin-33,ST2,Lentivirus vector,Inflammation,Collagen,Pulmonary fibrosis 【DOI】http://dx.doi.org/10.1016/j.intimp.2015.11.015 【影响因子(IF)】5.1 【出版期刊】《International Immunopharmacology》 【产品原文引用】 Hydroxyproline assay The hydroxyproline level was used to examine the content of collagen in the lung homogenates. The lungs were harvested 28 days after treatment and homogenized in PBS (pH 7.2–7.4). The supernatant was collected and aliquot after centrifuged at 3000 g for 15 min under 4 °C.The concentration of hydroxyproline was detected with Mouse Hyp kit (Jinma Biological Technology Co. Ltd.,Shanghai, China) as the specification.