什么是BrdU：BrdU是另外一种胸腺嘧啶核苷类似物。它也能够在细胞增殖时期代替胸腺嘧啶（T）渗入正在复制的DNA分子，通过基于EdU与Apollo荧光染料的特异性反应检测DNA复制活性，通过检测EdU标记便能准确地反映细胞的增殖情况。
BrdU的用途：
可代替胸腺嘧啶在DNA合成期（S期），活体注射或细胞培养加入，而后利用与Apollo荧光染料特异性反应检测细胞增殖情况。同时结合其它细胞标记物，双重染色，可判断增殖细胞的种类，增殖速度，对研究细胞动力学有重要意义。
BrdU的应用：
这类碱基替代物可以标记细胞分裂产生的时间点。因此，分时掺入BrdU和EdU可以做双标染色观察到不同的时间分裂的细胞。比如：一天给药BrdU，第二天给药EdU，之后染色观察到，BrdU阳性的细胞就代表了一天的，类似的EdU代表第二天。
参考文献：
【文献标题】Extracellular Vesicles Derived from Adipose Mesenchymal Stem Cells Regulate the Phenotype of Smooth Muscle Cells to Limit
Intimal Hyperplasia
【作者单位】上海交通大学(Shanghai Jiao Tong University)
【文献中引用产品】
Mouse BrdU ELISA Kit
【关键词】Extracellular vesicles . Adipose-derived
mesenchymal stem cells .Vein grafts .Vascular smoothmuscle
cells . Proliferation . Migration
【DOI】10.1007/s10557-015-6630-5
【影响因子(IF)】6.5
【出版期刊】《CARDIOVASCULAR DRUGS AND THERAPY》
【产品原文引用】 Two days later, cell proliferation was evaluated by measuring DNA synthesis with acolorimetric bromodeoxyuridine (BrdU) ELISA kit (Shanghai JinMa Laboratory ａnd Equipment Company, Shanghai, China) according to the manufacturer’sinstructions using a microplate reader (Molecular Devices,Sunnyvale, CA) to measure the absorbance.
【文献标题】Tissue factor pathway inhibitor gene transfer prevents vascular smooth muscle cell proliferation by interfering with the MCP-3/CCR2 pathway
【作者单位】哈尔滨医科大学( Harbin Medical University)
【文献中引用产品】
Mouse BrdU ELISA Kit
Mouse tumor necrosis factor α (TNF- α) ELISA kit
【DOI】doi:10.1038/labinvest.2015.106
【影响因子(IF)】6.7
【出版期刊】《Laboratory Investigation》
【产品原文引用】
Cell Proliferation StudiesVSMC proliferation was analyzed via measuring DNA synthesis with a colorimetric bromodeoxyuridine (BrdU) ELISA kit according to the manufacturer’s instructions (Shanghai JinMa Laboratory ａnd Equipment Company,Shanghai, China). Briefly, VSMCs were seeded in 24-well plates at a density of 5 × 104 cells/well. Three days after gene transfer, the cells were stimulated with recombinant mouse TNF-α (10 ng/ml) for 24 or 48 h, after which the cell culture supernatants were collected ａnd centrifuged at 12 000 g for 15 min at 4 °C; the extracted supernatants were immediately frozen at ? 80 °C until use. Absorbance values at 450 nm were determined using an ELISA reader (Bio-Rad, CA, USA).
原创作者：上海elisa生物技术有限公司
